The Effect of Plant Secondary Metabolites on Lipid Peroxidation and Eicosanoid Pathway

© 2012 Mimica-Dukić et al., licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Effect of Plant Secondary Metabolites on Lipid Peroxidation and Eicosanoid Pathway


Introduction
Inflammation, free radical damage and oxidative stress have become major health issues in recent years and the subject of plenty of research. These processes are implicated in cancer [1], cardiovascular diseases [2], multiple sclerosis [3], diabetes mellitus [4], Alzheimer's and Parkinson's diseases [5], rheumatoid arthritis [6], premature aging [7] and almost any other degenerative condition. Reactive oxygen species (ROS), which are involved in these physiological functional changes, are often either by-products of the normal cellular processes or are formed by action of exogenous factors -xenobiotics, ionizing radiation, stress, pathogens etc. Overproduction of ROS leads to oxidative stress, with biomolecules, including lipids, proteins and nucleic acids undergoing oxidative alterations.

Lipid peroxidation
Lipid peroxidation (LP) -oxidative degradation of polyunsaturated fatty acids caused by ROS -is responsible for degradation of membrane lipids resulting in cell damage and formation of many toxic products. LP is a free radical chain reaction where three major steps -the initiation, propagation and termination -can be recognised (Figure 1.).
In initiation phase, highly reactive hydroxyl radical, formed in Fenton reaction, abstracts hydrogen atom in α position relative to the polyunsaturated fatty acid double bond. This results in the formation of fatty acid radical, highly unstable, short-lived intermediate that stabilises by abstracting hydrogen from another chemical species, or reacts with triplet oxygen to generate different radical species, including fatty acid peroxyl radical. In the termination step peroxyl radicals transform into nonradical compounds -hydrocarbons, aldehydes, alcohols, volatile ketones and lipid polymers, some of which are harmful ( Figure  1.) [8,9]. Organism uses a number of endogenous antioxidants, such as glutathione, α-lipoic acid, coenzyme Q10, bilirubin and antioxidant enzymes (glutathione peroxidase, catalase, superoxide dismutase), to protect itself from oxidative stress. When they are insufficient, it becomes necessary to introduce exogenous antioxidants. Most of these compounds are primarily taken into the body by food and are predominantly of herbal origin (phenolics, carotenoids, terpenoids and vitamins -ascorbic acid and tocopherol). Herbal antioxidants exhibit their activity through a wide variety of mechanisms, such as inhibition of oxidising enzymes, chelation of transition metals, transfer of hydrogen or single electron to radicals, singlet oxygen deactivation, or enzymatic detoxification of ROS [8]. They can stop LP process in either initiation or propagation step. Herbal antioxidants have become subjects of growing interest and targets of numerous scientific research. However, enormous number of plant species is still waiting to be investigated in this manner and explored as potential medical drugs or dietary supplements.

Eicosanoid pathway
In addition to direct detrimental effects on biomolecules and cellular structures, lipid peroxidation is also involved in biosynthesis of eicosanoids -arachidonic acid metabolites serving as inflammatory mediators. Arachidonic acid, released from phospholipids by action of phospholipase A, can be converted into these products by three different pathways: cyclooxygenase, leading to the formation of prostanoids (prostaglandins and thromboxanes), lipoxygenase, where leukotrienes and certain mono-, di-and trihydroxy COX-catalysed transformation of arachidonic acid starts with tyrosyl radical generation through Tyr 385 oxidation by heme in COX active site. Formed tyrosyl radical abstracts hydrogen from C-13 of arachidonic acid. In subsequent steps, free electron migration, reaction with oxygen yielding peroxyl radical, and cyclisation reactions give PGG2 which is converted to PGH2 through the action of peroxidase (Px) (Figure 3.) [11].
COX-1 is expressed constitutively in different tissues, blood monocytes and platelets, and is involved in normal cellular homeostasis. In contrast, COX-2 may be induced by a series of pro-inflammatory stimuli and its role in the progress of inflammation, fever and pain has been known [12]. Furthermore, COX-2 has been targeted in many cancers including: colon cancer, colorectal cancer, breast cancer, gliomas, prostate cancer, esophageal carcinoma, pancreatic cancer, lung carcinoma, gastric carcinoma, ovarian cancer, Kaposi's sarcoma and melanoma [13].
In lipoxygenase branch of arachidonic acid metabolism, there are three types of lipoxygenases, termed 5-, 12-and 15-lipoxygenase. In 12-lipooxigenase (12-LOX) pathway, the first reaction is abstraction of hydrogen from C-10 of arachidonic acid, which includes reduction of Fe 3+ to Fe 2+ in enzyme active site. This results in the formation of arachidonic acid radical, which than reacts with oxygen and generates 12-hydroperoxyeicosatetraenoic acid (12-HPETE). Finally, formation of 12(S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoic acid (12-HETE) is catalysed by glutathione peroxidase (GPx), whereby glutathione (GSH) is oxidised to GS-SG (Figure 3.) [14,15]. 12-HETE is implicated in regulation of platelet aggregation, angiogenesis, as well as the progression of several human diseases like various cancers, rheumatoid arthritis and psoriasis [13,16,17]. Also, 12-HETE is known to take part in the metastatic cascade as a crucial intracellular signalling molecule which activates protein kinase C and mediates the biological functions of growth factors and cytokines.
Nowadays, there is a great need for a new anti-inflammatory compounds with minimal side effects. Natural products, especially phenolics frequently consumed in a diet of plant origin, are known to have great anti-inflammatory potential considering inhibition of COX and LOX enzymes [18,19]. Thus, screening of plants for COX/LOX inhibitory activity, followed by effect-guided fractionation, can be a useful tool for discovering new secondary biomolecules with anti-inflammatory potential. Although plant species widely used in traditional medicine may be a good starting point, there is also a vast number of currently unexplored species of unknown composition and activity. Therefore, this study included the poorly investigated plant species classified into four families -Alliaceae, Cupressaceae, Plantaginaceae and Polygonaceae -wild growing in Serbia, that are part of our continuing research [20][21][22][23][24][25][26][27][28][29]. Most of these species are used in diet or used in folk medicine for healing various disorders.
Considering the fact that lipid peroxidation is involved in an inflammation process, the aim of this study was to compare antioxidant activity (more specifically, the ability to inhibit lipid peroxidation) of the selected plant extracts with their ability to inhibit production of particular arachidonic acid metabolites and to correlate these activities with the phenolic and flavonoid content. Air-dried and grounded plant material (30 g) (whole plants of Allium sp., needles and cones of Juniperus sp., aerial parts of Plantago sp. and herbs and rhizomes of Rumex sp.) was extracted by maceration with 80% aqueous methanol (8 mL per 1 g of drug) during 72 h at room temperature. After filtration, the solvent was evaporated to dryness under reduced pressure. All raw extracts except those of Allium sp. and of Rumex sp. rhizomes were resuspended in hot distilled water (to a final concentration of approx. 1 g/mL), washed exhaustively with petroleum ether (fraction 40-60 °C) to remove nonpolar pigments, and evaporated to dryness under vacuum.

Plant material and extract preparation
Dried extracts were dissolved in 80% aqueous methanol and DMSO for evaluation of the antioxidant and anti-inflammatory activity, respectively, to obtain 300 mg/mL stock solutions.

Determination of total phenolic content
Total phenolic content was determined according to method of Singleton et al. [30], modified for 96-well microplates. Gallic acid was used as a standard for calibration curve construction. Thirty microliters of each extract or standard solution was added to 150 μL of 0.1 mol/L Folin-Ciocalteu reagent and after 10 min mixed with 120 μL of sodium carbonate (7.5%). The same mixture, with solvent instead of extract, was used as a blank. Absorbance at 760 nm was read after 2 h. The phenolics concentration was determined by using the calibration curve of gallic acid. The total phenolics value was expressed as milligrams of gallic acid equivalents per gram of dry weight (dw).

Determination of total flavonoid content
The aluminium chloride spectrophotometric method described by Chang et al. [31] and modified for 96-well microplates, was used for determination of of the total flavonoid content. Quercetin was used as a standard to prepare a calibration curve. The reaction mixture was comprised of 30 μL of the extract or standard solution, 90 μL of methanol, 6 μL of 10% aluminium chloride (substituted with distilled water in blank probe), 6 μL of 1 mol/L potassium acetate and 170 μL of distilled water. Absorbance at 415 nm was measured after 30 min. Flavonoid content was calculated according to the standard calibration curve and are expressed in milligrams of quercetin equivalents per gram of dw.

Lipid peroxidation
There are several methods for measuring the ability of plant extracts to inhibit lipid peroxidation [8]. Some of the methods are based on monitoring of malondialdehyde (MDA), a degradation product of polyunsaturated fatty acids peroxidation. Possible ways for quantification of MDA are GC-FID after derivatisation, HPLC with DAD or fluorimetric detector and spectrophotometric method. The latter is based on the reaction of MDA with thiobarbituric acid (TBA) and it is commonly used both in in vitro and in vivo studies. Formation of the red-coloured MDA-TBA adduct is measured at 532 nm. Different substrates can be used in this test: lecithin liposomes, free fatty acids, LDL and body fluids [8,32]. Also, a few different initiators of LP, such as ionizing radiation, chemical agentsmetal ions, free radicals and metalloproteins may be used [8].
In this study, we used spectrophotometric TBA assay [28,33] for evaluation the ability of extracts to inhibit LP. Linseed oil, used as a source of polyunsaturated fatty acids (69.7 % linolenic, 13.5 % linoleic acid, as determined by GC-MS), was obtained from linseed by Soxhlet extraction. Oil was added to 0.067 mol/L phosphate buffer, pH 7.4, in the presence of 0.25% Tween-80 to obtain a 0.035% suspension and sonicated for 1 hour. This suspension (3.0 mL) was mixed with 20 μL of FeSO4 (4.58 mmol/L), 20 μL of ascorbic acid (87 μmol/L), and 20 μL of extract (or solvent in control); 3.0 mL of phosphate buffer and 20 μL of extract were added in the blank probe. After incubation at 37 °C for 1 hour, 0.2 mL 3.72% EDTA was added to all samples followed by 2 mL of an aqueous mixture containing TBA (3.75 mg/mL), HClO4 (1.3%), and trichloroacetic acid (0.15 g/mL). Reaction mixtures were heated at 100 °C for 15 min, cooled, centrifuged at 1600 g for 15 min, and absorbance was measured at 532 nm. All samples and control were made in triplicate. IC50 values were determined from inhibition vs. concentration plots.

Anti-inflammatory activity
There are a great number of different in vitro methods used for estimation of inhibitory activity of COX and LOX enzymes including a number of commercial kits. Some assays, as a source of enzymatic activity, include native or recombinant enzymes, animals or human origin, while others use different cell lines that express desirable activities. Arachidonic acid is often added in reaction medium and in some cases is radio labelled. Induction of inflammatory respond also differs, and in most cases is performed by bacterial lipopolysaccharide, various cytokines and tumour necrosis factor. Different techniques are used for detection of enzymatic activity, such as different chromatographic techniques (TLC, HPLC-UV), as well as EIA [19,34].
In this study, COX-1 and 12-LOX inhibitory activity was investigated using ex vivo assay according to modified method of Safayhi et al. [29,35]. Intact cells (human platelets) were used as a source of COX-1 and 12-LOX enzymes. Arachidonic acid metabolites (12-HHT and 12-HETE) were determined by use of LC-MS/MS technique [29].
An aliquot of human platelet concentrate (viable but outdated for medical use) containing 4·10 8 cells was suspended in buffer (0.137 mol/L NaCl, 2.7 mmol/L KCl, 2.0 mmol/L KH2PO4, 5.0 mmol/L Na2HPO4 and 5.0 mmol/L glucose, pH 7.2) to obtain final volume of 2 mL. This mixture was slowly stirred at 37 °C for 5 min. Subsequently, 0.1 mL of extracts or standard compounds solutions in DMSO (concentration ranging from 10.0 to 200.0, 0.156 to 5.0 and 0.01 to 0.6 mg/ml for extracts, quercetin and aspirin, respectively) and 0.1 mL of calcimycin (Calcium Ionophore A23187, 125 μmol/L in DMSO) were added and incubated for 2 min at 37 °C, with moderate shaking. The exact volume of extract in control and calcimycin in blank probe were substituted with solvent (DMSO). Thereafter, 0.3 mL of CaCl2 aqueous solution (16.7 mmol/L), substituted with water in blank probe, was added and the mixture was incubated for further 5 min at 37 °C with shaking. Acidification with cold 1% aqueous formic acid (5.8 mL) to pH 3 terminated the reaction. If gel formation occurred, vortexing was applied before mixing with the acid. Prostaglandin B2 (50 μL of 6 μg/mL solution in DMSO) was added as internal standard, and extraction of products was done with mixture of chloroform and methanol (1:1, 8.0 mL) with vigorous vortexing for 15 min. After centrifugation at 7012 × g for 15 min at 4 °C, organic layer was separated, evaporated to dryness, dissolved in methanol (0.5 mL), filtered and used for further LC-MS/MS analysis. All samples and control were made in triplicate.
Test for estimation of the anti-inflammatory activity, applied in our research, has a lot of advantages. Firstly, the advantage is avoidance of the undesirable in vivo tests on experimental animals. Even though the exact anti-inflammatory activity can be validated only through in vivo tests, creating in vitro assays, in which physiological conditions similar to in vivo assays are used, can provide valuable information about inhibitory potential of the compounds tested. Platelets are a suitable cell system for testing inhibitory activity, because they can provide physiological cell conditions and possibility to examine the inhibition of both enzymes at the same time. Secondly, for determination of the formed metabolites as a measure of level of inhibition of COX and LOX activity, LC-MS/MS technique was used. LC-MS/MS provided a highly sensitive and specific detection of desirable metabolites within a short analysis time [29].

Statistical analysis
Percent of lipid peroxidation inhibition achieved by different concentration of extracts was calculated by the following equation: I(%) = (A0−A)/A0 × 100, where A0 was the absorbance of the control reaction and A was the absorbance of the examined samples, corrected for the value of blank probe. Percent of COX-1 and 12-LOX inhibition achieved by different concentrations of extracts was calculated by the following equation: I(%) = 100 × (R0−R)/R0, where R0 and R were response ratios (metabolite peak area divided by internal standard peak area) in the control reaction and in the examined samples, respectively. Both R and R0 were corrected for the value of blank probe. For both assays, corresponding inhibitionconcentration curves were drawn using Origin software, version 8.0 (Origin Labs) and IC50 values (concentration of extract that inhibited lipid peroxidation and COX-1 and 12-LOX metabolites formation by 50%) were determined. All of the results were expressed as mean ± SD of three replicates. Correlation analyses were done using Statistica software version 6 (StatSoft). Concentrations of total phenolics and the total flavonoids were used as independent variables, while inhibitory activities towards LP and 12-HETE synthesis (expressed as 1/IC50) were used as dependent variables. Due to a wide range of values, loglog plots were applied. Pearson's correlation coefficients were calculated.

Results
In this study, we tested the effect of 17 taxa from four families (Alliaceae, Cupressaceae, Plantaginaceae and Polygonaceae) on lipid peroxidation and metabolism of arachidonic acid. The total phenolic and flavonoid contents were determined in the plant extracts, as well. All results are presented in Figures 4, 5, 6 and 9.
Among all tested samples, herb and rhizome extracts of Rumex species were the most potent inhibitors of LP (0.009-0.047 mg/mL). Slightly lower activity was shown by herb extracts of Plantago species (0.025-0.178 mg/mL), while extracts of cones and needles of Juniperus species, as well as the whole plant extracts of Allium species expressed a much lower activity (0.117-0.887 and 0.68-1.986 mg/mL, respectively).   Total phenolic content in examined genera decreased in following order: Rumex, Juniperus, Plantago and Allium, with R. patentia and R. crispus extracts being by far the richest in phenolics (550 and 527 mg eq. gallic acid per 1 g dw, respectively). Regarding the content of the total flavonoids, significant intrageneric variations were observed, hence it was not possible to point out a genus with the highest content. Flavonoids were the most abundant in J. foetidissima needles extract (60 mg quercetin eq per 1 g dw), and scarcest in A. rhodopeum (0.2 mg quercetin eq per 1 g dw).
Plant phenolics present in extracts can counteract lipid peroxidation in either initiation or propagation step. Possible mechanisms are chelation of transition metals (Fe 2+ ), reduction of Fe 3+ and neutralisation of lipid peroxidation radical intermediates by transfer of hydrogen or single electron [8]. An attempt was made to correlate the observed lipid peroxidation inhibitory activity (given as 1/IC50) with the content of the total phenolics and total flavonoids. The corresponding plots are given in Figure 7. and Figure 8.
A high degree of correlation was observed between the lipid peroxidation inhibitory activity and the total phenolic content (r = 0.7713), while correlation with the total flavonoids content was not established (r = 0.1410). Thus, flavonoids do not contribute to the total observed activity to a significant extent. The lower activity of flavonoids, compared to other phenolics, can be explained through Porter's polar paradox. Namely, flavonoids present in the extracts investigated were predominantly in glycosylated form and thus highly polar. It is demonstrated that compounds of lower polarity are more effective in polar reaction media, such as oil-in-water emulsion used in our experiments, since they exhibit their activity at oil-water interface [36]. In addition, hydrogen atom donation ability of flavonoids decrease with glycosylation, especially if the most active hydroxyl groups (C-3, C-3' or C-4') are occupied by carbohydrate moiety [37,38].
Regarding the eicosanoid pathway, Rumex and Plantago species showed, on average, very high 12-LOX inhibitory effect, with IC50 in range 0.75-3.59 mg/mL (Figure 9.). While Juniperus and Allium also exhibited dose-dependent inhibition of 12-HETE production, their IC50 values were higher, ranging from 1.45 mg/mL to 11.04 mg/mL.   Moreover, certain inhibitory activity of the examined extracts towards COX-1 was also confirmed in the anti-inflammatory assay applied in this research. Determined IC50 values ranged from 0.34 to 8.00 mg/mL, with no genus exhibiting significantly higher activity than others. However, the results are not shown due to lack of correlation with the total phenolics and flavonoid content. This is in agreement with our previous findings [27].
The exact mechanism of COX and LOX enzymes inhibition by natural products is still not fully elucidated. However, bearing in mind their structures and chemical properties, several mechanisms could be speculated. Both pathways of arachidonic acid metabolism include free radical reactions (Figure 3.). Due to their radical scavenging activity or reducing properties, many plant natural products can interfere with reactions catalysed by COX and LOX [39]. They can neutralise radical intermediates, thus terminating the reaction. In addition, they can reduce Fe 3+ ion that is a part of active site of both enzymes and is necessary for initiation reaction. Some natural products, including acetylenes, can bind covalently to enzymes and inhibit them irreversibly [19]. Also, in inhibition of LOX, isoprenyl moiety of some phenolics and terpenoid backbone structure could play an important role. Prenylated phenolics are usually more hydrophobic than conventional ones. Terpenoids are also mostly non-polar compounds. These characteristics suggest an easy penetration through the cell membrane and their good 12-lipoxygenase inhibitory properties [19]. Finally, the COX or LOX activity can be decreased by suppressing their transcription by phenolics [18], although this effect is observable only in long-duration experiments.
To identify the compound class responsible for the observed 12-LOX-inhibitory activity, 1/IC50 was correlated with the content of the total phenolics and flavonoids. The corresponding plots are given in Figure 10. and Figure 11. As with the lipid peroxidation, the correlation between the content of the total phenolics and 12-LOX-inhibitory activity was also observed, although slightly weaker, with Pearson's correlation coefficient r = 0.6037. At the same time, no relationship was found between the total flavonoids content and 12-HETE production (r = 0.2825). Bearing in mind a good correlation of 12-LOX inhibition with the phenolic content, and the lack thereof with the flavonoid content, it is possible that only small phenolic molecules, but not the voluminous flavonoid glycosides, can enter 12-LOX active site and exhibit the inhibitory effect there. Thus, the observed differences in the total phenolics and the total flavonoids effects can at least partially be explained by steric hindrance. The differences in COX and LOX inhibition (LOX inhibition being correlated with phenolic content) can be attributed to the differences in reaction mechanism and active site threedimensional structure. Namely, both mechanisms include abstraction of hydrogen from arachidonic acid leading to formation of radical species. However, hydrogen acceptor in COX is tyrosyl radical (formed through tyrosine oxidation by Fe 3+ ) while in LOX, electron is transferred directly to Fe 3+ . Phenolics from plant extracts could reduce Fe 3+ to Fe 2+ , thus inactivating both enzymes. However, the presence of tyrosyl residue in COX active site could provide steric protection and prevent phenolics from approaching the Fe 3+ ion.
Finally, the correlation between the ability of extracts to counteract lipid peroxidation and inhibit production of 12-HETE is shown in Figure 12. High correlation coefficient (r = 0.6819) suggests that extracts with a high inhibitory effect on LP, also represent potent inhibitors of 12-LOX pathway. Methods for examination of anti-inflammatory activity are labourintensive, expensive and sometimes involve ethical issues due to the usage of laboratory animals. Therefore, in vitro measurement of lipid peroxidation inhibition and the total phenolic content could be a useful tool for screening of plant extracts with a potential antiinflammatory activity. Figure 11. Correlation between total flavonoid content and 12-LOX inhibitory activity To summarise, the most of the examined species expressed high lipid peroxidation and antiinflammatory activity, especially Rumex and Plantago species. High inhibitory activity of these species towards 12-LOX pathway makes them good candidates for further research taking into account role of this enzyme in cancer development. A good correlation was found between total phenolic content of 23 investigated plant extracts and their ability to inhibit lipid peroxidation and 12-LOX pathway. Consequently, LP inhibitory activity was also highly correlated with 12-LOX inhibition. Thus it can serve as indicator for preliminary selection of plant extracts further to be tested for anti-inflammatory activity by expensive and time-consuming methods.